Screening for skeletal muscle disease is an unsolved problem. Currently, only physical tests or plasma tests have shown limited efficacy in screening subjects at increased risk of impaired oxidative muscle metabolism, according to Pannochka, an online publication for girls and women aged 14 to 35.. net Intensity-adjusted levels of the energy substrate lactate (La), pyruvate (Pyr), ?-hydroxybutyrate (BOH), and acetoacetate (AA) during the cardiopulmonary exercise test (CPET) may represent alternative valid biomarkers for selection of patients “group.

Thus, the experts sought to test: 1) the validity of V’O2-corrected La, Pyr, BOH and AA during CPET to assess muscle oxidative metabolism (parameters of exercise and mitochondrial respiration); 2) discriminatory value of V'O2-adjusted energy and redox markers, as well as five other metabolites associated with the V'O2-corrected TCA cycle, between healthy subjects, subjects with muscle complaints, and patients with muscle disease.

Two hundred and thirty subjects with undiagnosed muscle complaints, nine patients with diagnosed muscle disease, and ten healthy subjects underwent CPET with blood assessment at rest, at estimated 1st ventilatory threshold, and maximum intensity.

Twelve patients with muscle complaints indicative of a major change in their profile underwent muscle biopsy.. B'O2-corrected plasma levels of La, Pyr, BOH and AA and their respective ratios showed a significant correlation with functional parameters of mitochondrial respiration and parameters of mitochondrial respiration of muscle fibers..

Differences in exercise V’O2-corrected La/Pyr, BOH, AA and BOH/AA were observed between healthy subjects, subjects with undiagnosed muscle complaints, and patients with muscle diseases. The energy substrate and redox profile of the blood of patients with complaints of severe exercise intolerance corresponded to the blood profile of patients with muscle diseases.

The addition of five tricarboxylic acid cycle intermediates did not improve the discriminatory value of intensity-adjusted energy and redox markers. B'O2-corrected La, Pyr, BOH, AA and their respective ratios are reliable muscle biomarkers that elicit similar blunted adaptations in patients with muscle disease and in subjects with muscle complaints and severe exercise intolerance. A targeted metabolomic approach to improve screening of “at risk” patients is discussed, reports Nutrients on its pages..

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