Barkley's goal in injury time brought guests three points.
Leicester hosted Aston Villa in the fifth round of the English Championship and lost with a minimum score.
The only goal of the match was scored by Ross Barkley already in stoppage time for the fight.
Leicester - Aston Villa 0: 1 Goal: Barkley, 90 + 1 Leicester: Schmeichel - Castagne, Fofana, Evans, Justin - Thielemans, Prat (Maddison, 68), Mendy (Chowdury, 78) - Barnes, Perez, Iheanacho (Slimani,.
Aston Villa: Martinez - Cash, Mings, Konsa, Targett - Barkley, McGinn, Grealish, Trezeguet (Traore, 81), Douglas Louis - Watkins.
Warnings: Perez, Thielemans, Castagne, Evans - McGin, Cash, Louis.